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Purification and characterization of TnsC, a Tn7 transposition protein that binds ATP and DNA.

The bacterial transposon Tn7 encodes five transposition genes tnsABCDE. We report a simple and rapid procedure for the purification of TnsC protein. We show that purified TnsC is active in and required for Tn7 transposition in a cell-free recombination system. This finding demonstrates that TnsC par...

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書目詳細資料
發表在:Nucleic Acids Res
Principais autores: Gamas, P, Craig, N L
格式: Artigo
語言:Inglês
出版: Oxford University Press 1992
在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312388/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1317955/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.10.2525
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