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Purification and characterization of TnsC, a Tn7 transposition protein that binds ATP and DNA.
The bacterial transposon Tn7 encodes five transposition genes tnsABCDE. We report a simple and rapid procedure for the purification of TnsC protein. We show that purified TnsC is active in and required for Tn7 transposition in a cell-free recombination system. This finding demonstrates that TnsC par...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1992
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| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC312388/ https://ncbi.nlm.nih.gov/pubmed/1317955 |
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