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Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.
A Hot Start Polymerase Chain Reaction (PCR) entails the withholding of at least one reagent from the reaction mixture until the reaction tube temperature has reached 60-80 degrees C. Hot Start amplification with an AmpliWax vapor barrier uses a layer of solid wax to separate the retained reagent(s)...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1992
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| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312262/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1579465/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.7.1717 |
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