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Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.
A Hot Start Polymerase Chain Reaction (PCR) entails the withholding of at least one reagent from the reaction mixture until the reaction tube temperature has reached 60-80 degrees C. Hot Start amplification with an AmpliWax vapor barrier uses a layer of solid wax to separate the retained reagent(s)...
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| Main Authors: | , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1992
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| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC312262/ https://ncbi.nlm.nih.gov/pubmed/1579465 |
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