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Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.

A Hot Start Polymerase Chain Reaction (PCR) entails the withholding of at least one reagent from the reaction mixture until the reaction tube temperature has reached 60-80 degrees C. Hot Start amplification with an AmpliWax vapor barrier uses a layer of solid wax to separate the retained reagent(s)...

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Dettagli Bibliografici
Autori principali: Chou, Q, Russell, M, Birch, D E, Raymond, J, Bloch, W
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1992
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC312262/
https://ncbi.nlm.nih.gov/pubmed/1579465
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