Resolution and purification of free primase activity from the DNA primase-polymerase alpha complex of HeLa cells.
DNA primase activity has been resolved from a purified DNA primase-polymerase alpha complex of HeLa cells by hydrophobic affinity chromatography on phenylSepharose followed by chromatography on hexylagarose. This procedure provides a good yield (55%) of DNA primase that is free from polymerase alpha...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1986
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| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311871/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3786132/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.21.8467 |
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