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Resolution and purification of free primase activity from the DNA primase-polymerase alpha complex of HeLa cells.

DNA primase activity has been resolved from a purified DNA primase-polymerase alpha complex of HeLa cells by hydrophobic affinity chromatography on phenylSepharose followed by chromatography on hexylagarose. This procedure provides a good yield (55%) of DNA primase that is free from polymerase alpha...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Principais autores: Vishwanatha, J K, Baril, E F
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1986
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311871/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3786132/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.21.8467
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