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Resolution and purification of free primase activity from the DNA primase-polymerase alpha complex of HeLa cells.

DNA primase activity has been resolved from a purified DNA primase-polymerase alpha complex of HeLa cells by hydrophobic affinity chromatography on phenylSepharose followed by chromatography on hexylagarose. This procedure provides a good yield (55%) of DNA primase that is free from polymerase alpha...

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Detalhes bibliográficos
Main Authors: Vishwanatha, J K, Baril, E F
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC311871/
https://ncbi.nlm.nih.gov/pubmed/3786132
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