Prevention of guanine modification and chain cleavage during the solid phase synthesis of oligonucleotides using phosphoramidite derivatives.
Phosphoramidite reagents can phosphitylate guanine bases at the O6-position during solid phase synthesis and serious chain cleavage occurs if the base phosphitylation is not eliminated before the iodine/water oxidation step. This can be accomplished by blocking the O6-position with a 2-cyanoethyl pr...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1986
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311657/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3748816/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.16.6453 |
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