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Prevention of guanine modification and chain cleavage during the solid phase synthesis of oligonucleotides using phosphoramidite derivatives.

Phosphoramidite reagents can phosphitylate guanine bases at the O6-position during solid phase synthesis and serious chain cleavage occurs if the base phosphitylation is not eliminated before the iodine/water oxidation step. This can be accomplished by blocking the O6-position with a 2-cyanoethyl pr...

詳細記述

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書誌詳細
出版年:Nucleic Acids Res
主要な著者: Pon, R T, Usman, N, Damha, M J, Ogilvie, K K
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 1986
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311657/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3748816/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.16.6453
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