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Prevention of guanine modification and chain cleavage during the solid phase synthesis of oligonucleotides using phosphoramidite derivatives.

Phosphoramidite reagents can phosphitylate guanine bases at the O6-position during solid phase synthesis and serious chain cleavage occurs if the base phosphitylation is not eliminated before the iodine/water oxidation step. This can be accomplished by blocking the O6-position with a 2-cyanoethyl pr...

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Bibliografske podrobnosti
Main Authors: Pon, R T, Usman, N, Damha, M J, Ogilvie, K K
Format: Artigo
Jezik:Inglês
Izdano: 1986
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC311657/
https://ncbi.nlm.nih.gov/pubmed/3748816
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