Prevention of guanine modification and chain cleavage during the solid phase synthesis of oligonucleotides using phosphoramidite derivatives.

Phosphoramidite reagents can phosphitylate guanine bases at the O6-position during solid phase synthesis and serious chain cleavage occurs if the base phosphitylation is not eliminated before the iodine/water oxidation step. This can be accomplished by blocking the O6-position with a 2-cyanoethyl pr...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Pon, R T, Usman, N, Damha, M J, Ogilvie, K K
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 1986
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311657/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3748816/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.16.6453
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