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Isolation of viable antigen-specific CD8(+) T cells based on membrane-bound tumor necrosis factor (TNF)-α expression
Current technology to isolate viable cytokine-producing antigen-specific primary human T cells is limited to bi-specific antibody capture systems, which suffer from limited sensitivity and high background. Here, we describe a novel procedure for isolating antigen-specific human T cells based on thei...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2011
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3116017/ https://ncbi.nlm.nih.gov/pubmed/21501617 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2011.04.003 |
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