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Application of a High-throughput Fluorescent Acetyltransferase Assay to Identify Inhibitors of Homocitrate Synthase

Homocitrate synthase (HCS) catalyzes the first step of L-lysine biosynthesis in fungi by condensing acetyl-Coenzyme A and 2-oxoglutarate to form 3R-homocitrate and Coenzyme A. Due to its conservation in pathogenic fungi, HCS has been proposed as a candidate for antifungal drug design. Here we report...

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Bibliografiske detaljer
Main Authors: Bulfer, Stacie L., McQuade, Thomas J., Larsen, Martha J., Trievel, Raymond C.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3115995/
https://ncbi.nlm.nih.gov/pubmed/21073853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.11.004
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