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Determination of Real-Time Efflux Phenotypes in Escherichia coli AcrB Binding Pocket Phenylalanine Mutants Using a 1,2′-Dinaphthylamine Efflux Assay

To evaluate the importance of phenylalanine residues for substrate transport in the Escherichia coli efflux pump protein AcrB, we subjected Phe-to-Ala binding pocket mutants to a real-time efflux assay with the novel near-infrared lipophilic membrane probe 1,2′-dinaphthylamine (1,2′-DNA). All mutati...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Bohnert, Jürgen A., Schuster, Sabine, Szymaniak-Vits, Magdalena, Kern, Winfried V.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Public Library of Science 2011
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3115983/
https://ncbi.nlm.nih.gov/pubmed/21698261
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0021196
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