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Site-Directed Mutagenesis Reveals Putative Substrate Binding Residues in the Escherichia coli RND Efflux Pump AcrB

The Escherichia coli multidrug efflux pump protein AcrB has recently been cocrystallized with various substrates, suggesting that there is a phenylalanine-rich binding site around F178 and F615. We found that F610A was the point mutation that had the most significant impact on substrate MICs, while...

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Detalhes bibliográficos
Main Authors: Bohnert, Jürgen A., Schuster, Sabine, Seeger, Markus A., Fähnrich, Eva, Pos, Klaas M., Kern, Winfried V.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2593224/
https://ncbi.nlm.nih.gov/pubmed/18849422
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00912-08
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