Visualization of oligonucleotide probes and point mutations in interphase nuclei and DNA fibers using rolling circle DNA amplification

Rolling circle amplification (RCA) is a surface-anchored DNA replication reaction that can be exploited to visualize single molecular recognition events. Here we report the use of RCA to visualize target DNA sequences as small as 50 nts in peripheral blood lymphocytes or in stretched DNA fibers. Thr...

Full description

Bibliographic Details

Published in:	Proc Natl Acad Sci U S A
Main Authors:	Zhong, Xiao-bo, Lizardi, Paul M., Huang, Xiao-hua, Bray-Ward, Patricia L., Ward, David C.
Format:	Artigo
Language:	Inglês
Published:	National Academy of Sciences 2001
Subjects:	Biological Sciences
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC31158/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11274414/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.061026198
Tags:	Add Tag No Tags, Be the first to tag this record!