Visualization of oligonucleotide probes and point mutations in interphase nuclei and DNA fibers using rolling circle DNA amplification

Rolling circle amplification (RCA) is a surface-anchored DNA replication reaction that can be exploited to visualize single molecular recognition events. Here we report the use of RCA to visualize target DNA sequences as small as 50 nts in peripheral blood lymphocytes or in stretched DNA fibers. Thr...

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Detaylı Bibliyografya
Asıl Yazarlar: Zhong, Xiao-bo, Lizardi, Paul M., Huang, Xiao-hua, Bray-Ward, Patricia L., Ward, David C.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: The National Academy of Sciences 2001
Konular:
Biological Sciences
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC31158/
https://ncbi.nlm.nih.gov/pubmed/11274414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.061026198
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