Global gene disruption in human cells to assign genes to phenotypes

Insertional mutagenesis in a haploid background can lead to complete disruption of gene function(1). Here we generate a population of human cells that contain insertions in >98% of their expressed genes. We established Phenotypic Interrogation via Tag Sequencing (PhITSeq) as a method to examine m...

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Detalhes bibliográficos
Main Authors: Carette, Jan E., Guimaraes, Carla P., Wuethrich, Irene, Blomen, Vincent A., Varadarajan, Malini, Sun, Chong, Bell, George, Yuan, Bingbing, Muellner, Markus K., Nijman, Sebastian M., Ploegh, Hidde L., Brummelkamp, Thijn R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3111863/
https://ncbi.nlm.nih.gov/pubmed/21623355
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.1857
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