Identification of host cell factors required for intoxication through use of modified cholera toxin

We describe a novel labeling strategy to site-specifically attach fluorophores, biotin, and proteins to the C terminus of the A1 subunit (CTA1) of cholera toxin (CTx) in an otherwise correctly assembled and active CTx complex. Using a biotinylated N-linked glycosylation reporter peptide attached to...

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Detalhes bibliográficos
Main Authors: Guimaraes, Carla P., Carette, Jan E., Varadarajan, Malini, Antos, John, Popp, Maximilian W., Spooner, Eric, Brummelkamp, Thijn R., Ploegh, Hidde L.
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 2011
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Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3257576/
https://ncbi.nlm.nih.gov/pubmed/22123862
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201108103
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