Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

BACKGROUND: Accurate interpretation of quantitative PCR (qPCR) data requires normalization using constitutively expressed reference genes. Ribosomal RNA is often used as a reference gene for transcriptional studies in E. coli. However, the choice of reliable reference genes has not been systematical...

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Main Authors: Zhou, Kang, Zhou, Lihan, Lim, Qing 'En, Zou, Ruiyang, Stephanopoulos, Gregory, Too, Heng-Phon
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2011
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3110127/
https://ncbi.nlm.nih.gov/pubmed/21513543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-12-18
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