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Normalization with genes encoding ribosomal proteins but not GAPDH provides an accurate quantification of gene expressions in neuronal differentiation of PC12 cells

BACKGROUND: Gene regulation at transcript level can provide a good indication of the complex signaling mechanisms underlying physiological and pathological processes. Transcriptomic methods such as microarray and quantitative real-time PCR require stable reference genes for accurate normalization of...

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Détails bibliographiques
Auteurs principaux: Zhou, Lihan, Lim, Qing-En, Wan, Guoqiang, Too, Heng-Phon
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2010
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2831847/
https://ncbi.nlm.nih.gov/pubmed/20113474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-11-75
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