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Variations at the Semiconserved Glycine in the IQ Domain Consensus Sequence Have a Major Impact on Ca(2+)-Dependent Switching in Calmodulin–IQ Domain Complexes()
We have replaced the semiconserved Gly in the IQ domain consensus sequence with Ala, Arg, or Met in a reference sequence and determined how this affects its complexes with calmodulin. The K(d) for the Ca(2+)-free reference complex is 2.4 ± 0.3 μM. The Ala and Arg replacements increase this to 5.4 ±...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3105379/ https://ncbi.nlm.nih.gov/pubmed/19954189 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi901695p |
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