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Enhancement of the cleavage rates of DNA-armed hammerhead ribozymes by various divalent metal ions.
In order to characterize structure-function relationships, the kinetic behavior of chimeric RNA/DNA ribozyme was compared with that of all RNA ribozyme. Determined kcat values were proven to represent the chemical-cleavage step and not the product-dissociation step. In agreement with the finding by...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1993
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310531/ https://ncbi.nlm.nih.gov/pubmed/7506830 |
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