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Enhancement of the cleavage rates of DNA-armed hammerhead ribozymes by various divalent metal ions.

In order to characterize structure-function relationships, the kinetic behavior of chimeric RNA/DNA ribozyme was compared with that of all RNA ribozyme. Determined kcat values were proven to represent the chemical-cleavage step and not the product-dissociation step. In agreement with the finding by...

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Publicado en:Nucleic Acids Res
Autores principales: Sawata, S, Shimayama, T, Komiyama, M, Kumar, P K, Nishikawa, S, Taira, K
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 1993
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310531/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7506830/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.24.5656
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