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Enhancement of the cleavage rates of DNA-armed hammerhead ribozymes by various divalent metal ions.

In order to characterize structure-function relationships, the kinetic behavior of chimeric RNA/DNA ribozyme was compared with that of all RNA ribozyme. Determined kcat values were proven to represent the chemical-cleavage step and not the product-dissociation step. In agreement with the finding by...

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Autors principals: Sawata, S, Shimayama, T, Komiyama, M, Kumar, P K, Nishikawa, S, Taira, K
Format: Artigo
Idioma:Inglês
Publicat: 1993
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC310531/
https://ncbi.nlm.nih.gov/pubmed/7506830
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