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Quantitative evaluation of refolding conditions for a disulfide-bond-containing protein using a concise (18)O-labeling technique
A concise method was developed for quantifying native disulfide-bond formation in proteins using isotopically labeled internal standards, which were easily prepared with proteolytic (18)O-labeling. As the method has much higher throughput to estimate the amounts of fragments possessing native disulf...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Wiley Subscription Services, Inc., A Wiley Company
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3104239/ https://ncbi.nlm.nih.gov/pubmed/21500299 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.637 |
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