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Detecting native folds in mixtures of proteins that contain disulfide bonds
High-throughput in vitro refolding of proteins that contain disulfide bonds, for which soluble expression is particularly difficult, is severely impeded by the absence of effective methods for detecting their native forms. We demonstrate such a method, which combines mass spectrometry with mild redu...
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| Autori principali: | , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2008
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2602968/ https://ncbi.nlm.nih.gov/pubmed/18278035 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt1380 |
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