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Detecting native folds in mixtures of proteins that contain disulfide bonds

High-throughput in vitro refolding of proteins that contain disulfide bonds, for which soluble expression is particularly difficult, is severely impeded by the absence of effective methods for detecting their native forms. We demonstrate such a method, which combines mass spectrometry with mild redu...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Narayan, Mahesh, Welker, Ervin, Zhai, Huili, Han, Xuemei, Xu, Guoqiang, McLafferty, Fred W, Scheraga, Harold A
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2008
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC2602968/
https://ncbi.nlm.nih.gov/pubmed/18278035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt1380
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