Purification and biochemical characterisation of the EcoR124 type I modification methylase.
Large scale purification of the type I modification methylase EcoR124 has been achieved from an over-expressing strain by a two step procedure using ion-exchange and heparin chromatography. Pure methylase is obtained at a yield of 30 mg per gm of cell paste. Measurements of the molecular weight and...
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| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1992
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310352/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1741244/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.2.179 |
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