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Purification and biochemical characterisation of the EcoR124 type I modification methylase.

Large scale purification of the type I modification methylase EcoR124 has been achieved from an over-expressing strain by a two step procedure using ion-exchange and heparin chromatography. Pure methylase is obtained at a yield of 30 mg per gm of cell paste. Measurements of the molecular weight and...

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Bibliografiske detaljer
Main Authors: Taylor, I, Patel, J, Firman, K, Kneale, G
Format: Artigo
Sprog:Inglês
Udgivet: 1992
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC310352/
https://ncbi.nlm.nih.gov/pubmed/1741244
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