Tritium labeling of antisense oligonucleotides by exchange with tritiated water.
We describe a simple, efficient, procedure for labeling oligonucleotides to high specific activity (< 1 x 10(8) cpm/mumol) by hydrogen exchange with tritiated water at the C8 positions of purines in the presence of beta-mercaptoethanol, an effective radical scavenger. Approximately 90% of the starti...
Αποθηκεύτηκε σε:
| Εκδόθηκε σε: | Nucleic Acids Res |
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| Κύριοι συγγραφείς: | , , , , , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
Oxford University Press
1993
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| Διαθέσιμο Online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309879/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8367289/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.16.3737 |
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