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Tritium labeling of antisense oligonucleotides by exchange with tritiated water.
We describe a simple, efficient, procedure for labeling oligonucleotides to high specific activity (< 1 x 10(8) cpm/mumol) by hydrogen exchange with tritiated water at the C8 positions of purines in the presence of beta-mercaptoethanol, an effective radical scavenger. Approximately 90% of the sta...
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| Главные авторы: | , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
1993
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| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309879/ https://ncbi.nlm.nih.gov/pubmed/8367289 |
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