Tritium labeling of antisense oligonucleotides by exchange with tritiated water.

We describe a simple, efficient, procedure for labeling oligonucleotides to high specific activity (< 1 x 10(8) cpm/mumol) by hydrogen exchange with tritiated water at the C8 positions of purines in the presence of beta-mercaptoethanol, an effective radical scavenger. Approximately 90% of the starti...

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Dades bibliogràfiques

Publicat a:	Nucleic Acids Res
Autors principals:	Graham, M J, Freier, S M, Crooke, R M, Ecker, D J, Maslova, R N, Lesnik, E A
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 1993
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309879/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8367289/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.16.3737
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