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Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments into plasmids.
The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lox from the lox/Cre recombinase system of bacteriophage P1. There are two distinct stages. Firstly, vector and fragment DNAs are ligated intermolecularly under conditions of macromolecular crowding (15...
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| Autore principale: | |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
1993
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| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309212/ https://ncbi.nlm.nih.gov/pubmed/8451184 |
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