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Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments into plasmids.

The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lox from the lox/Cre recombinase system of bacteriophage P1. There are two distinct stages. Firstly, vector and fragment DNAs are ligated intermolecularly under conditions of macromolecular crowding (15...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Hovedforfatter: Boyd, A C
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1993
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309212/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8451184/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.4.817
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