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Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments into plasmids.

The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lox from the lox/Cre recombinase system of bacteriophage P1. There are two distinct stages. Firstly, vector and fragment DNAs are ligated intermolecularly under conditions of macromolecular crowding (15...

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Bibliografiska uppgifter
Huvudupphovsman: Boyd, A C
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1993
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC309212/
https://ncbi.nlm.nih.gov/pubmed/8451184
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