Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments into plasmids.
The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lox from the lox/Cre recombinase system of bacteriophage P1. There are two distinct stages. Firstly, vector and fragment DNAs are ligated intermolecularly under conditions of macromolecular crowding (15...
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| Publicado en: | Nucleic Acids Res |
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| Autor principal: | |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1993
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| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309212/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8451184/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.4.817 |
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