Turbo cloning: a fast, efficient method for cloning PCR products and other blunt-ended DNA fragments into plasmids.
The method uses a novel plasmid vector, p9lox5, containing a site-specific recombination sequence lox from the lox/Cre recombinase system of bacteriophage P1. There are two distinct stages. Firstly, vector and fragment DNAs are ligated intermolecularly under conditions of macromolecular crowding (15...
সংরক্ষণ করুন:
| প্রকাশিত: | Nucleic Acids Res |
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| প্রধান লেখক: | |
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
Oxford University Press
1993
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| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309212/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8451184/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.4.817 |
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