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Investigating Inner Sphere Reorganization via Secondary Kinetic Isotope Effects in the C–H Cleavage Reaction Catalyzed by Soybean Lipoxygenase: Tunneling in the Substrate Backbone as well as the Transferred Hydrogen
This work describes the application of NMR to the measurement of secondary deuterium (2° (2)H) and carbon-13 ((13)C) kinetic isotope effects (KIES) at positions 9 to 13 within the substrate linoleic acid (LA) of soybean lipoxygenase-1. The KIEs have been measured using linoleic acid labeled with eit...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3090704/ https://ncbi.nlm.nih.gov/pubmed/21192631 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja1050742 |
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