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A hydrogen-bonding network is important for oxidation and isomerization in the reaction catalyzed by cholesterol oxidase
Cholesterol oxidase is a flavoenzyme that catalyzes the oxidation and isomerization of 3β-hydroxysteroids. Structural and mutagenesis studies have shown that Asn485 plays a key role in substrate oxidation. The side chain makes an NH⋯π interaction with the reduced form of the flavin cofactor. A N485D...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3089011/ https://ncbi.nlm.nih.gov/pubmed/19923719 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0907444909037421 |
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