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A hydrogen-bonding network is important for oxidation and isomerization in the reaction catalyzed by cholesterol oxidase

Cholesterol oxidase is a flavoenzyme that catalyzes the oxidation and isomerization of 3β-hydroxysteroids. Structural and mutagenesis studies have shown that Asn485 plays a key role in substrate oxidation. The side chain makes an NH⋯π interaction with the reduced form of the flavin cofactor. A N485D...

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Hlavní autoři: Lyubimov, Artem Y., Chen, Lin, Sampson, Nicole S., Vrielink, Alice
Médium: Artigo
Jazyk:Inglês
Vydáno: International Union of Crystallography 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3089011/
https://ncbi.nlm.nih.gov/pubmed/19923719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0907444909037421
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