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A Synthetic Approach to Stop-Codon Scanning Mutagenesis
A general combinatorial mutagenesis strategy using common DMT-protected mononucleotide phosphoramidites and a single orthogonally-protected trinucleotide phosphoramidite (Fmoc-TAG) was developed to scan a gene with the TAG amber stop codon with complete synthetic control. In combination with stop-co...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3085015/ https://ncbi.nlm.nih.gov/pubmed/21452871 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja106894g |
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