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High-throughput thermal scanning: a general, rapid dye-binding thermal shift screen for protein engineering
The low stability of natural proteins often limits their use in therapeutic, industrial and research applications. The scale and throughput of methods such as circular dichroism, fluorescence spectroscopy and calorimetry severely limit the number of variants that can be examined. Here we demonstrate...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2701314/ https://ncbi.nlm.nih.gov/pubmed/19292479 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja8049063 |
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