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Characterization and Comparison of Disulfide Linkages and Scrambling Patterns in Therapeutic Monoclonal Antibodies - Using LC-MS with Electron Transfer Dissociation

The disulfides in three monoclonal antibodies (mAb), the anti-HER2, anti-CD11a, and GLP-1 with IgG4-Fc fusion protein, were completely mapped by LC-MS with the combination of ETD and CID fragmentation. In addition to mapping the 4 inter- and 12 intra-chain disulfides (total 16), the identification o...

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Autors principals: Wang, Yi, Lu, Qiaozhen, Wu, Shiaw-Lin, Karger, Barry L., Hancock, William S.
Format: Artigo
Idioma:Inglês
Publicat: 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3082428/
https://ncbi.nlm.nih.gov/pubmed/21428412
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac200128d
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