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Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of an inositol monophosphatase family protein (SAS2203) from Staphylococcus aureus MSSA476

The gene product of the sas2203 ORF of Staphylococcus aureus MSSA476 encodes a 30 kDa molecular-weight protein with a high sequence resemblance (29% identity) to tetrameric inositol monophosphatase from Thermotoga maritima. The protein was cloned, expressed, purified to homogeneity and crystallized....

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Autors principals: Bhattacharyya, Sudipta, Dutta, Debajyoti, Ghosh, Ananta Kumar, Das, Amit Kumar
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2011
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3080153/
https://ncbi.nlm.nih.gov/pubmed/21505244
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111003496
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