Cloning, purification, crystallization and preliminary crystallographic analysis of the human histone deacetylase sirtuin 1

Human sirtuin 1 is a member of the histone deacetylase family and is involved in cellular aging, tumourigenesis and cellular metabolism. Recombinant sirtuin 1 comprising residues 140–747 was crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to 3.4 Å resolution and b...

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Autori principali: Holbourn, Kenneth P., Lloyd, Matthew D., Thompson, Andrew S., Threadgill, Michael D., Acharya, K. Ravi
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2011
Soggetti:
Crystallization Communications
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3080150/
https://ncbi.nlm.nih.gov/pubmed/21505241
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111003277
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