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Purification, crystallization and preliminary crystallographic analysis of histone lysine demethylase NO66 from Homo sapiens

NO66 is a JmjC domain-containing histone demethylase with specificity towards histone H3 methylated on both Lys4 and Lys36 in vitro and in vivo. A fragment of NO66 lacking the N-terminal 167 amino-acid residues was overexpressed in Escherichia coli, purified and crystallized using the sitting-drop v...

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Détails bibliographiques
Auteurs principaux: Zhou, Xing, Tao, Yue, Wu, Minhao, Zhang, Dandan, Zang, Jianye
Format: Artigo
Langue:Inglês
Publié: International Union of Crystallography 2012
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388916/
https://ncbi.nlm.nih.gov/pubmed/22750859
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911201740X
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