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Purification, crystallization and preliminary crystallographic analysis of histone lysine demethylase NO66 from Homo sapiens
NO66 is a JmjC domain-containing histone demethylase with specificity towards histone H3 methylated on both Lys4 and Lys36 in vitro and in vivo. A fragment of NO66 lacking the N-terminal 167 amino-acid residues was overexpressed in Escherichia coli, purified and crystallized using the sitting-drop v...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
International Union of Crystallography
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3388916/ https://ncbi.nlm.nih.gov/pubmed/22750859 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911201740X |
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