Učitavanje...
Cloning, purification, crystallization and preliminary crystallographic analysis of SecA from Enterococcus faecalis
The gene coding for SecA from Enterococcus faecalis was cloned and overexpressed in Escherichia coli. In this protein, the lysine at position 6 was replaced by an asparagine in order to reduce sensitivity towards proteases. The modified protein was purified and crystallized. Crystals diffracting to...
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| Glavni autori: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
International Union of Crystallography
2006
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2243102/ https://ncbi.nlm.nih.gov/pubmed/16754988 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106017544 |
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