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Cloning, expression, purification, crystallization and initial crystallographic analysis of the preprotein translocation ATPase SecA from Thermus thermophilus

The Thermus thermophilus gene encoding the preprotein translocation ATPase SecA was cloned and expressed and the purified protein was crystallized by the hanging-drop vapour-diffusion technique in two different space groups P3(1(2))21 (a = b = 168.6, c = 149.8 Å) and P6(1(5))22 (a = b = 130.9, c = 5...

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Autors principals: Vassylyeva, Marina N., Mori, Hiroyuki, Tsukazaki, Tomoya, Yokoyama, Shigeyuki, Tahirov, Tahir H., Ito, Koreaki, Vassylyev, Dmitry G.
Format: Artigo
Idioma:Inglês
Publicat: International Union of Crystallography 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2242882/
https://ncbi.nlm.nih.gov/pubmed/16946477
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106030843
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