Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription elongation factors GreB from Escherichia coli and Gfh1 from Thermus thermophilus

The Escherichia coli gene encoding the transcription cleavage factor GreB and the Thermus thermophilus gene encoding the anti-GreA transcription factor Gfh1 were cloned and expressed and the purified proteins were crystallized by the sitting-drop vapor-diffusion technique. The GreB and Gfh1 crystals...

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Detalhes bibliográficos
Main Authors: Perederina, Anna A., Vassylyeva, Marina N., Berezin, Igor A., Svetlov, Vladimir, Artsimovitch, Irina, Vassylyev, Dmitry G.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2005
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1401493/
https://ncbi.nlm.nih.gov/pubmed/16511259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105040297
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