Purification, crystallization and preliminary X-ray diffraction of SecDF, a translocon-associated membrane protein, from Thermus thermophilus

Thermus thermophilus has a multi-path membrane protein, TSecDF, as a single-chain homologue of Escherichia coli SecD and SecF, which form a translocon-associated complex required for efficient preprotein translocation and membrane-protein integration. Here, the cloning, expression in E. coli, purifi...

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Bibliographic Details
Main Authors: Tsukazaki, Tomoya, Mori, Hiroyuki, Fukai, Shuya, Numata, Tomoyuki, Perederina, Anna, Adachi, Hiroaki, Matsumura, Hiroyoshi, Takano, Kazufumi, Murakami, Satoshi, Inoue, Tsuyoshi, Mori, Yusuke, Sasaki, Takatomo, Vassylyev, Dmitry G., Nureki, Osamu, Ito, Koreaki
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2006
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2222563/
https://ncbi.nlm.nih.gov/pubmed/16582489
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106007779
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