The Effect of Formaldehyde Fixation on RNA: Optimization of Formaldehyde Adduct Removal

Formalin-fixed, paraffin-embedded tissues generally provide low yields of extractable RNA that exhibit both covalent modification of nucleic acid bases and strand cleavage. This frustrates efforts to perform retrospective analyses of gene expression using archival tissue specimens. A variety of cond...

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Bibliographic Details
Main Authors: Evers, David L., Fowler, Carol B., Cunningham, Brady R., Mason, Jeffrey T., O'Leary, Timothy J.
Format: Artigo
Language:Inglês
Published: American Society for Investigative Pathology 2011
Subjects:
Regular Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3077733/
https://ncbi.nlm.nih.gov/pubmed/21497290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2011.01.010
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