The Effect of Formaldehyde Fixation on RNA: Optimization of Formaldehyde Adduct Removal

Formalin-fixed, paraffin-embedded tissues generally provide low yields of extractable RNA that exhibit both covalent modification of nucleic acid bases and strand cleavage. This frustrates efforts to perform retrospective analyses of gene expression using archival tissue specimens. A variety of cond...

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Detalhes bibliográficos
Main Authors: Evers, David L., Fowler, Carol B., Cunningham, Brady R., Mason, Jeffrey T., O'Leary, Timothy J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Investigative Pathology 2011
Assuntos:
Regular Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3077733/
https://ncbi.nlm.nih.gov/pubmed/21497290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2011.01.010
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