Antigen Retrieval Causes Protein Unfolding: Evidence for a Linear Epitope Model of Recovered Immunoreactivity

Antigen retrieval (AR), in which formalin-fixed paraffin-embedded tissue sections are briefly heated in buffers at high temperature, often greatly improves immunohistochemical staining. An important unresolved question regarding AR is how formalin treatment affects the conformation of protein epitop...

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Detalhes bibliográficos
Main Authors: Fowler, Carol B., Evers, David L., O’Leary, Timothy J., Mason, Jeffrey T.
Formato: Artigo
Idioma:Inglês
Publicado em: SAGE Publications 2011
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Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3201144/
https://ncbi.nlm.nih.gov/pubmed/21411808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1369/0022155411400866
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